感染、炎症、修复2012，Vol.13Issue(2)：79-82,封3,5.
中药复方托毒生髓方治疗慢性骨髓炎的实验研究
Experimental study of Chinese herbal compound Tuodu Shengsui concoction (托毒生髓方) in treatment of chronic osteomyelitis
摘要
Abstract
Objectives To observe the effect of Chinese herbal compound Tuodu Shengsui concoction (托毒生髓方 ) in treatment of chronic osteomyelitis in rabbits. Methods: Sixty rabbits were randomly divided into four groups. Rabbits in normal control group were fed ordinary fodder. The model of chronic osteomyelitis was replicated in rabbits of the other 3 groups: an aperture measuring 0. 5 cm×1.0 cm in the right tibia of rabbits was formed by osteotomy, and 5 ml sodium morrhuate and 0. 5 ml Staphylococcus aureus suspension (5×106 CFU/ml) were injected, then the aperture was sealed with bone wax. The model was verified by X-ray image 3 weeks later. In the Tuodu Shengsui concoction group the concoction was gavaged (5 ml, twice a day), and the wounds were irrigated (5 ml, twice a day or four times a day) with the Tuodu Shengsui concoction. Wounds of gentamicin group and model group were irrigated with either 10 ml of diluted gentamicin or normal saline (twice a day or four times a day) respectively. All animals were treated for 2 months, then blood leukocyte count, the levels of serum lyso-zyme activity and bone X-ray picture of the animals were observed on the 20-, 40-, 60-days after treatment Results: Compared with those in the normal control group, the blood leukocyte count, and the levels of serum lyso-zyme activity were decreased significantly at each time points in the model group and the gentamicin group(P< 0. 01), while they became higher in the gentamicin group than those in the model group(P<0. 05 or P<0. 01). In Tuodu Shengsui concoction group, they were increased significantly and reached the levels of normal control group on 60th day after treatment (P>0.05). On 60th day, the X-ray picture in the Tuodu Shengsui concoction group was superior to that of the other groups. On 40th day, X-ray showed bone abscess and high-density shadow of sequestrum in the model group, narrowing or disappearance of marrow cavity in the gentamicin group. However, in the Tuodu Shcngsui concoction group there was alleviation of inflammatory reaction and remodeling of bone structure. Conclusions. Tuodu Shengsui concoction is helpful in controlling inflammation, recovery of bone structure and recanalization of medullary fasciculus. The mechanism might be related to improvement of immune function and repair of bone injury.关键词
慢性骨髓炎/托毒生髓方/免疫指标/X线/动物实验Key words
Chronic osteomyelitis/ Tuodu Shengsui concoction/ Leukocyte/ Lysozyme/X-ray引用本文复制引用
李素那,钱丽旗,郑彬丽,于洋,高飞,杨丁友,武冰,孙大铭..中药复方托毒生髓方治疗慢性骨髓炎的实验研究[J].感染、炎症、修复,2012,13(2):79-82,封3,5.基金项目
军队中医药专项基金资助项目(10ZY247) （10ZY247）
