广东药学院学报2012，Vol.28Issue(3)：268-272,5.DOI:10.3969/j.issn.1006-8783.2012.03.010
类模板分子印迹整体柱识别人血清中甲氨蝶呤的研究
Study on recognition of methotrexate in human serum by molecularly imprinted monolithic column with an analogue template employed
摘要
Abstract
Objective To prepare a molecularly imprinted monolithic column for selectively enriching methotrexate( MTX) in human serum in order to facilitate the RP-HPLC analysis. Methods Melamine was employed as an analogue template to prepare the molecularly imprinted monolithic column by in-situ polymerization. Methacrylic acid was used as a function monomer and ethylene glycol dimethacrylate as a cross linker during the polymerization process. The recognition ability of the imprinted monolithic column towards MTX was evaluated by HPLC analysis. Results The matrix impurities in human serum could be eliminated and MTX was retained on MIP column during consecutive elution with methanol-water (1:9) and acetonitrile-water-acetic acid(96:3.5:0.5). Finally,MTX was obtained through the elution process of methanol for HPLC analysis. Conclusion The prepared MIP monolithic column showed good selective recognition capacity towards MTX under the optimal conditions of mobile phase,which may be applied in online or off-line manner to selectively enrich MTX in human serum, and clear the matrix impurities to provide good MTX fraction for RP-HPLC analysis.关键词
分子印迹聚合物/整体柱/类模板/选择性富集/高效液相色谱法/甲氨蝶呤/人血清Key words
molecularly imprinted polymer/ monolithic column/ analogue template/ selective enrichment/ high performance chromatography/ methotrexate/ human serum分类
医药卫生引用本文复制引用
张红武,史美丽,李雪美,陈星宇,卢绮雯,蔡冰..类模板分子印迹整体柱识别人血清中甲氨蝶呤的研究[J].广东药学院学报,2012,28(3):268-272,5.基金项目
中国科学院活体分析化学重点实验室开放基金资助项目(ACL201202) （ACL201202）
