广东药学院学报2012，Vol.28Issue(3)：299-302,4.DOI:10.3969/j.issn.1006-8783.2012.03.017
GC法测定鸦胆子油口服干乳颗粒剂中油酸与亚油酸的含量
Determination of oleic and linoleic acid in the oral dry emulsion granules of Brucea javanica oil by GC
张琳 1方成玲 2叶伟文 2杨展平 2金描真2
作者信息
- 1. 广东省妇幼保健院 药学部,广东广州510010
- 2. 广东药学院 药科学院,广东广州510006
- 折叠
摘要
Abstract
To develop a method for the determination of oleic and linoleic acid in the oral dry emulsion granules of Brucea javanica oil. Methods The method of capillary gas chromatography was used in this experiment. Fatty acids were derivatized with the methanol solution of boron trifluoride and analyzed by GC. The method was performed on the CP-WAX 52CB(30 m ×0. 32 mm ×0. 50 μm) capillary column at 205℃ ,the detector was FID at 300 ℃ ,the injector' temperature was 250 ℃ .and 1 μL was injected. The split ratio was 5:1. Phenyl benzoate was selected for internal standard. Results Under the above chromatographic conditions,oleic and linoleic acid had good linear relationship in their concentration range of 0.009 028-0.180 56 mg · mL-1(r=0.999 9 ) and 0.0045 13-0.090 26 mg · mL-1(r = 0.999 9), and the average recovery were 98.82% and 98.91% , respectively. Conclusion This method was sensitive,accurate and reproducible,which may provide the basis for the quality evaluation and control of the oral dry emulsion granules of Brucea javanica oil.
关键词
鸦胆子油乳/脂肪酸/气相色谱法/颗粒剂/油酸/亚油酸Key words
Brucea javanica oil emulsions/ fatty acid/ gas chromatography/ granules/ oleic acid/ linoleic?acid分类
医药卫生引用本文复制引用
张琳,方成玲,叶伟文,杨展平,金描真..GC法测定鸦胆子油口服干乳颗粒剂中油酸与亚油酸的含量[J].广东药学院学报,2012,28(3):299-302,4.
