摘要
Abstract
Objective: To explore the effect of aspirin on inducible nitric oxide synthase(iNOS) content induced by high concentrations of glucose and insulin in vascular smooth muscle cells (VSMCs), and to analyze the relationship between changes in of the levels of iNOS in VSMCs and NO in the cell culture medium in the state of high glucose and high insulin. Methods: Primary cultures of VSMCs were derived from the thoracic aortae of Wistar rats. The VSMCs were induced by addition of high concentrations of glucose or insulin, and were divided into four groups: a control group (CON), a high glucose group (HG), a high insulin group (Hi), a high glucose plus high insulin group (HGI), an aspirin + HG group (AHG; 2.50 mmol/L), an aspirin +HI group (AHI; 2.50 mmol/
L ), and an aspirin +HGI (AHGI; aspirin at 2.50 mmol/L (each n=6). Results: High glucose and high insulin both could reduce the iNOS content in VSMCs (P<0.05), high glucose plus high insulin could reduce the iNOS content in VSMCs significantly (P<0.01). Aspirin could promote the content of iNOS in VSMCs induced by high glucose and high insulin respectively P<0.05), and significantly promoted it induced by high glucose plus high insulin(P<0.0l). High glucose, high insulin, high glucose plus high insulin all could reduce the content of NO in cell culture medium (P<0.05), but aspirin could promote the content of NO in the previous 3 groups (P<0.05). There was no completely correlation in the reduction of iNOS in VSMCs and NO in the cell culture medium in HGI group (r=0.778), and the increase of iNOS in VSMCs and NO in the cell culture in AHGI group(r= 0.632). Conclusion: In the state of high glucose plus high insulin, aspirin could promote the content of iNOS in VSMCs and the content of NO in the cell cuture medium, but the iNOS content in VSMCs may not be the only factor that effect the NO content in the cell culture medium.关键词
阿司匹林/高糖/胰岛素/血管平滑肌细胞/诱导型一氧化氮合酶/一氧化氮Key words
aspirin/ high glucose/ insulin/ vascular smooth muscle cells/ inducible nitric oxide synthase/ nitrogen oxide
