国际病理科学与临床杂志2012，Vol.32Issue(3)：200-204,5.
MiR-122下调Bcl-2蛋白家族增强BEL-7402/FU细胞对氟尿嘧啶的敏感性
MiR-122 increases sensitivity of BEL-7402/FU cells to FU via down-regulation of Bcl-2 family proteins
摘要
Abstract
Objective: To investigate the effect of miR-122 on sensitivity to fluorouratil (FU) in transfected BEL-7402/ FU cells. Methods: MiR-122 and negative empty expression vectors were constructed and stably transfected into BEL-7402/FU cells. Western blot was used to detect Bcl-2 and Bcl-XL protein expression in the miR-122 transfectant group(n=3), the negative empty vector transfectant group (?=3) and an untreated cell group(n=3). Drug sensitivity of the cells to FU was analyzed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: Compared with the negative empty vector transfectant group and the untreated cells group, the protein expression levels of Bcl-2, Bcl-XL in the stable miR-122 transfectant group were decreased (P<0.05). MTT results showed that after the incubation with FU, the miR-122 transfectant group had higher cell inhibitory rates than the negative empty vector transfectant group and the untreated cells group (P<0.05). Conclusion: MiR-122 can specifically down-regulate the expression of Bd-2 and Bcl-XL in BEL-7402/FU cells, which, in turn, increases spontaneous apoptosis and sensitivity to FU.关键词
微小RNA/Bcl-2/Bcl-XL/BEL-7402/氟尿嘧啶细胞/凋亡/氟尿嘧啶Key words
miRNA/ Bd-2/ Bcl-XL/ BEL-7402/fluorouracil cells/ apoptosis/ fluorouracil引用本文复制引用
殷杰,虞佳,杨晓燕,向琼,彭翔,唐惠芳,雷小勇..MiR-122下调Bcl-2蛋白家族增强BEL-7402/FU细胞对氟尿嘧啶的敏感性[J].国际病理科学与临床杂志,2012,32(3):200-204,5.基金项目
国家自然基金(30900625) （30900625）
湖南省教育厅项目(08A059) （08A059）
