南方农业学报2012，Vol.43Issue(4)：413-416,4.DOI:10.3969/j:issn.2095-1191.2012.04.413
鹤望兰八氢番茄红素合成酶基因克隆及表达分析
Cloning and expression of phytoene synthase gene in Strelitzia reginae
摘要
Abstract
[Objective]The present experiment was conducted to explore the function of Phytoene synthase (PSY) gene in regulating flower color formation in Slrelitzia regime. [Method]PSY segment involved in carotenoid biosynthesis was cloned from yellow flower sepals of Strelitzia reginae by homology sequence cloning using primers designed by conserved amino acid sequence of plant PSY gene. This segment was compared by sequence alignment and analyzed phyloge-netically. Expression characteristics of PSY gene in different organs and at different development stage of flower was analyzed using RT-PCR assays. [Result]The length of this segment was found 248 bp, encoding a 82 predicted amino acids. The accession number of this segment was registered in CenBank as JN887695. The predicted amino acid sequence shared high homologies with other PSY proteins in other plants, which was 90% with PSY of Allium sativum. It was preliminarily proved to be the target gene. PSY showed the highest transcript abundance in the yellow sepals or at early stage of flower development. [Conclusion]The PSY in Streiitaa reginae may regulate the forming of yellow sepals at the transcriptional level.关键词
鹤望兰/八氢番茄红素合成酶(PSY)/基因克隆/花色形成/表达分析Key words
Strelitzia reginae/ phytoene synthase/ gene cloning/ flower color formation/ expression分类
农业科技引用本文复制引用
樊荣辉,黄敏玲,林兵,钟淮钦..鹤望兰八氢番茄红素合成酶基因克隆及表达分析[J].南方农业学报,2012,43(4):413-416,4.基金项目
福建省科技重大专项项目(2010NZ0003-1) （2010NZ0003-1）
福建省农业科学院科技创新团队重点科研项目(CXTD2011-20) （CXTD2011-20）
