南方农业学报2012，Vol.43Issue(4)：417-420,4.DOI:10.3969/j:issn.2095-1191.2012.04.417
转拟南芥NPR1基因水稻253 T3代株系对水稻白叶枯病的抗性及其农艺性状表现
Agronomic traits and rice bacterial blight disease resistance in T3 generation lines of rice cultivar 253 over-expressing Arabidopsis thaliana NPR1 gene
摘要
Abstract
[ Objective ]The present experiment was conducted to attain transgenic Aiabidopsis thaliana NPR1 gene restorer rice line 253 with high resistance, and to compare the differences in agronomic traits among transgenic rice and wild type 253 to provide transgenic material for disease resistance breeding. [ Method ]Six T3 generation lines {253-3, 253-4, 253-7, 165, 166 and 167) of transgenic rice eultivar 253, over-expressing A. thaliana AtNPRI gene and wild type 253 were used for investigating their resistance against rice bacterial blight disease after inoculation for 14 days using leaf-cutting method at tillering stage and comparing their agronomic traits at harvesting stage. [Result]Six 13 generation lines of transgenic rice eultivar 253 showed stable rice bacterial blight disease resistance, and their lesion lengths were significantly shorter than that of non-transgenic wild 253. As compared to wild 253, the disease resistance of line 253-3 enhanced over 50%, and that of the other five lines, viz., 253-4, 253-7, 165, 166 and 167 enhanced over 90%. All of T3 generation lines showed normal growth and there were no significant regular changes in their agronomic traits, however, some agronomic traits showed significant increase or decrease compared to wild 253. The plant height, panicle length, effective panicles, primary rachis branches, total grains per panicle, full grains per panicle and yield per plant were significantly higher than that of non-transgenic 253. [Conclusion]A. thaliana NPR1 gene is good candidate gene for breeding broad disease resistance crops in the future, and transgenic line 166 could be used as new material in breeding.关键词
AtNPR1基因/转基因/恢复系253/抗病性/农艺性状Key words
AtNPR1/ transgenic/ restorer line 253/ disease resistance/ agronomic traits分类
农业科技引用本文复制引用
罗雪梅,罗荡平,曹启龙,冯家勋,段承杰..转拟南芥NPR1基因水稻253 T3代株系对水稻白叶枯病的抗性及其农艺性状表现[J].南方农业学报,2012,43(4):417-420,4.基金项目
广西自然科学基金项目(桂科自0007006) （桂科自0007006）
广西科技攻关项目(桂科攻0228019-4) （桂科攻0228019-4）
