南方农业学报2012，Vol.43Issue(4)：443-448,6.DOI:10.3969/j:issn.2095-1191.2012.04.443
鄂西北产猫眼草不同极性部位提取物抑菌活性评价
Evaluation on antifungal activity of different polar fractions of Enphorbia lunulata Bag obtained from Northwestern Hubei
摘要
Abstract
[Objective]Inhibitory effects of different polar fractions of Enphorbia lunulata Bag, collected from Northwestern Hubei, were observed on mycelial growth and spore germination in three vegetable pathogenic fungi in order to develop new plant fungicides. [ Method ]The whole herb of E. lunulata was extracted with ethanol, methanol, ethyl acetate and chloroform. And the antifungal activity of these extracts to Fusarium oxysporum fsp. cucumber Owen, Botrytis cinerea Pers, Sclerotium rolfsii Sacc was investigated by detecting the mycelium growth rate and the spore germination. [Result] Except weak inhibitory effect of methanol extract on B. cinerea and S. rolfsii, other different polar fractions showed strong inhibitory activity which increased with increasing concentration of extracts. Chloroform fraction showed the highest inhibition effects to F. oxysporium f.sp cucumber (EC30 for 0.007 g/mL); ethyl acetate fraction's inhibition effect was the highest in both the B. cinerea (EC30 for 0.008 g/mL) and S. rolfsii (EC50 for 0.011 g/mL). Inhibition of different polar fractions in E. lunulata on the above pathogenic fungi increased with increasing concentration. Similar inhibitory effects were observed in mycelial growth, that of methanol梕xtracting fractions were relatively weaker, but the fractions extracted with ethyl acetate and chloroform-extracting fractions were significantly stronger. The inhibitory efficiency of chloroform fraction to F. oxysporium f.sp cucumber (0.005 g/mL) was the highest; while both, the B. cinerea (EC50 for 0.005 g/mL) and S. rolfsii (EC50 for 0.004 g/mL) the ethyl acetate fraction was the highest. [Conclusion]All the different polar fractions of E. lunulata had inhibition on the tested pathogenic fungi, hence E. lunulata can be used for bio-fungicide development.关键词
猫眼草/有机溶剂/提取物/病原真菌/抑菌活性Key words
Enphorbia lunulata Bag/ extracts/ pathogenic fungi/ antiiungal activity分类
农业科技引用本文复制引用
余海忠,孙永林,廖雪义,王海燕..鄂西北产猫眼草不同极性部位提取物抑菌活性评价[J].南方农业学报,2012,43(4):443-448,6.基金项目
湖北省教育厅优秀中青年人才项目(Q20082504) （Q20082504）
湖北省襄阳市科技攻关项目(200517,2011GG1C10) （200517,2011GG1C10）
襄樊学院植物资源研究开发培育团队项目(Kypytd200901) （Kypytd200901）
