南方农业学报2012，Vol.43Issue(4)：511-514,4.DOI:10.3969/j:issn.2095-1191.2012.04.511
薄膜—均质法制备番茄红素脂质体的工艺研究
Preparation of lycopene liposome using membrane-homogeneous method
摘要
Abstract
[Objective]The experiment was conducted to enhance the bioavailability of lycopene by improving entrapment rate and reducing particle size. [ Method ] Lycopene liposome was prepared by membrane-homogeneous method. Entrapment rate was used as major index in single factor experiment and orthogonal test, where cholesterol/lecithin ratio, lycopene/lecithin ratio, rotating evaporation temperature and phosphate buffer solution were determined to obtain the optimal processing procedure. Furthermore, lipsome morphology and particle characteristics were recorded. [Result]The best processing parameters were observed as: cholesterol/lecithin ratio of 1:3, lycopene/lecithin ratio of 1:20, rotating e-vaporation temperature of 30 ℃ and phosphate buffer solution pH 7.0. Under the best technological conditions, entrapment rate of lycopene was 71.65% and the average particle size was 840 nm. [Conclusion]It was suggested that preparing lycopene lipsome using membrane-homogeneous method is simple and apt to controll. This method should be promoted in production due to its high entrapment rate, small particle and great stability.关键词
番茄红素/脂质体/薄膜—均质法/包封率Key words
lycopene/ liposome/ membrane-homogeneous method/ entrapment rate分类
化学化工引用本文复制引用
欧春凤,叶盛英,张静,韩笑..薄膜—均质法制备番茄红素脂质体的工艺研究[J].南方农业学报,2012,43(4):511-514,4.基金项目
国家自然科学基金项目(30871446) （30871446）
