广西医科大学学报2012，Vol.29Issue(3)：333-337,5.
雌激素对前成骨细胞胰岛素受体底物-1的影响
EFFECT OF ESTRADIOL ON THE EXPRESSION PROFILE OF IRS-1 DURING MC3T3-E1 CELLS DIFFERENTIATION
摘要
Abstract
Objective: To observe the effect of estradiol on the expression profile of insulin receptor sub-strate-1 (IRS-1) during murine preosteoblastic MC3T3-E1 cells differentiation. Methods: MC3T3-E1 cell was cultured and underwent intervention of 1 7j3-estradial. Cells -were collected respectively in days 0, 6, 12, 18, 24 and 30. Semi-quantitative RT-PCR -was used to measure the expression of mRNA of IRS-1. The expression of the proteins of IRS-1 was detected by Western blotting. Results: IRS-1 mRNA and protein levels gradually increased during differentiation of MC3T3-E1 cells. Estradiol increased IRS-1 mRNA and protein expressions in the -whole time course. Conclusion: IRS-1 may play an important role in the process of estradiol inducing bone turnover.关键词
MC3T3-E1细胞/胰岛素受体底物-1/17β-雌二醇Key words
murine preosteoblastic MC3T3-E1 cells/ insulin receptor substrate-1 / 17β-estradial分类
医药卫生引用本文复制引用
何玉玲,周后德,隋国良,谢辉,廖二元..雌激素对前成骨细胞胰岛素受体底物-1的影响[J].广西医科大学学报,2012,29(3):333-337,5.基金项目
国家自然科学基金资助项目(No.30400218) （No.30400218）
