广西植物2012，Vol.32Issue(3)：293-297,5.DOI:10.3969/j.issn.1000-3142.2012.05.002
中国板栗EST-SSR分子标记在栲树中的通用性分析
Transferability analysis of EST-SSR markers of Castanea mollissima to Castanopsis fargesii
摘要
Abstract
Simple sequence repeats(SSR),also known as microsatellite molecular markers,have been cross-amplified successfully in closely related species of the same genus and even across genera within the same family. In the present study, 124 primer pairs of polymorphic EST-SSR originally developed from Castanea mollissima were cross-amplified in Castanopsis fargesii. Results indicated that 42. 7% of Castanea mollssima EST-SSR primers were successfully cross-amplified in Castanopsis far gesii and 56. 6% were polymorphic. The genetic diversity of 4 populations of C. fargesii were investigated with polymorphic EST-SSRs, preliminary results showed that C. fargesii possessed high levels of genetic diversity(Na=6.105,Ho=0. 563,He-0.621). These polymorphic EST-SSR primers would provide a powerful tool for further investigation on population genetics of C. fargesii.关键词
中国板栗/栲树/EST-SSR/通用性Key words
Castanea mollissima/ Castanopsis fargesii / EST-SSR/ transferability分类
农业科技引用本文复制引用
李春,孙晔..中国板栗EST-SSR分子标记在栲树中的通用性分析[J].广西植物,2012,32(3):293-297,5.基金项目
国家自然科学基金(30871959,31170512) （30871959,31170512）
中国科学院生命科学领域基础前沿研究专项(KSCX2-EW-J-28) （KSCX2-EW-J-28）
大科学装置工程项目(2009-LSFGBOWS-01) （2009-LSFGBOWS-01）
