广西植物2012，Vol.32Issue(3)：298-303,292,7.DOI:10.3969/j.issn.1000-3142.2012.05.003
中日5个岛屿山茶种群遗传多样性研究
Study on population genetic diversity of Camellia japonica in 5 islands between China and Japan
摘要
Abstract
Camellia japonica is an important horticultural species, widely distributed in East-Asia. 5 populations of C. japonica collected from China and Japan were analyzed by ISSR markers to detect the genetic diversity. A total of 205 discernible loci were obtained from all populations using 20 primers. Of these loci ,89. 27% were polymorphic (PPB= 89. 27%), The average percentage of polymorphic loci (PPB= 72. 00%),Nei's gene diversity(HE = 0. 2743)and Shannon's Information Index(H=0. 4023),estimated by POPGENE,indicated that C. japonica had higher level genetic diversity than many other insular plant species. The coefficient gene differentiation (Gst) was 0. 2033, which showed more variation were from the individuals of the populations. According to Mantel Test (r= 0. 7989, P<0. 05) and UFGMA, insular geographical isolation have an important influence on genetic structure. In order to maintain genetic diversity and resource utilization of C. japonica that natural population should be protected in situ from human disturbance to facilitate its natural generation.关键词
山茶/ISSR/岛屿/遗传多样性/遗传结构Key words
Camellia japonica /ISSR/ island/ genetic diversity/ genetic structure分类
农业科技引用本文复制引用
林立,倪穗,李纪元,陈越,应震..中日5个岛屿山茶种群遗传多样性研究[J].广西植物,2012,32(3):298-303,292,7.基金项目
浙江省科技厅重点项目(2008C14065) （2008C14065）
宁波市国际科技合作项目(2010D10013) （2010D10013）
宁波市农村科技创新创业资金项目(2010C91026) （2010C91026）
国家国际科技合作项目(2011DFA30490) （2011DFA30490）
