广西植物2012，Vol.32Issue(3)：367-370,4.DOI:10.3969/j.issn.1000-3142.2012.05.017
菘蓝APX基因克隆及序列分析
Cloning and identification of APX gene from Isatis indigotica
摘要
Abstract
To construct the recombinant eukaryotic expression plasmid of pTZ57R/T/APX. APX gene was amplified from the genomic DNA of Isatis indigotica by polymerase chain reaction(PCR). APX gene was then inserted into pTZ57R/T eukaryote expression vector. The positive colonies were screened and identified by PCR and sequencing. The specific gene APX which was about 1 257 bp, was successfully amplified and pTZ57R/T-APX were constructed The DNA sequence of APX gene was as the same as the APX gene nucleotide sequences published in Genebank. pTZ57R/T-APX recombinant was successfully constructed And the construction provides the basis for the further study.关键词
菘蓝/APX基因/真核表达载体/基因克隆Key words
Isatis indigotica / APX gene/ eukaryote expression vector/ gene cloning分类
农业科技引用本文复制引用
孙晓东,李爱玲,韩立敏..菘蓝APX基因克隆及序列分析[J].广西植物,2012,32(3):367-370,4.基金项目
陕西省教育厅科研计划项目(09JK341) （09JK341）
陕西教育学院科研基金(11KJ006) （11KJ006）
