广西植物2012，Vol.32Issue(3)：371-376,6.DOI:10.3969/j.issn.1000-3142.2012.05.018
拟南芥成花调控LFY基因的选择性剪接
Alternative splicing of flowering regulatory gene LFY in Arabidopsis thaliana
摘要
Abstract
To study the expression of flowering regulatory gene LFY gene in Arabidopsis thaliana,we used reversetranscription-polymerase chain reaction and isolated three alternative splicing(AS)fragments named as LFY1239,LFY1263,and LFY1275 respectively.Sequence analysis confirmed that fragment LFY1263 contained an open reading frame of 1 263 bp,and was identical to the previously reported and predicted fragment,while LFY1239 lacked 36bp at the 3' end of the first exon,and LFY1275 had an additional 12bp derived from the 3' end of the first intron.Expression analysis showed that LFY1239 was only detected in rosette during the vegetative stage,while LFY1263 and LFY1275 presented in both floral organs and rosette during both vegetative and flowering stages.Furthermore,LFY1263 appeared to be the most abundant transcript.The expression ratio of LFY1275 to LFY1263 was higher in floral organs than in rosette leaves,which suggested that such ratio might be associated with the flowering regulation.关键词
LFY基因/成花转变/选择性剪接/差异表达Key words
LFY gene/ floral transition/ alternative splicing(AS)/ differential expression分类
生物科学引用本文复制引用
于丽霞,姚有林,武晓璐,汤晓倩,鄢波..拟南芥成花调控LFY基因的选择性剪接[J].广西植物,2012,32(3):371-376,6.基金项目
Supported by the National Natural Science Foundation of China(31060042,31160177) （31060042,31160177）
Yunnan Province and Minister Key Subject,University Key Lab and Share Platform （）
