广西植物2012，Vol.32Issue(3)：385-391,7.DOI:10.3969/j.issn.1000-3142.2012.05.021
秋水仙素对蚕豆胚根生长的影响及多倍体诱导效应分析
Influence of colchicine on radicle growth of Vicia faba and the polyploid inductive effect
摘要
Abstract
In order to test the optimum induction combination(time and concentration), and analyse the inductive effects of colchicine induced the radicle polyploid in Vicia faba. The radicle cells were treated with the colchicine of five concentrations (0.025%,0. 050%,0.100%,0.150% ,0. 200%,respectively) combining various culture time (12,24, 48,60 h,respectively). And the radicle cell were treated with distilled water simultaneously,which were taken as the control. The optimum induction combination induced by colchicine was confirmed by significant difference of the root tip inductivity and the radicle expanding ratio. And the chromosome number of radicle cells and stoma number of seedling were detected to identify the induction effect. The radicle amount and seeding height were analized subsequently. The optimum inductive condition was 48 h combining colchicine concentration of 0.100%. There were significant differences in stoma number between inducted time and inducted concentration, however, the radicle number and height of V. faba seedling showed significant difference in inductive concentration but no significant difference in culture time.关键词
多倍体/蚕豆/秋水仙素/胚根Key words
polyploid/ Vicia faba/colchicine/ radicle分类
生物科学引用本文复制引用
闫秋洁,杨琼..秋水仙素对蚕豆胚根生长的影响及多倍体诱导效应分析[J].广西植物,2012,32(3):385-391,7.基金项目
四川省教育厅青年基金(10ZB140) （10ZB140）
绵阳师范学院创新基金(Mnucx09012) （Mnucx09012）
