β-葡萄糖苷酶高产菌株的筛选及其性质研究OA北大核心CSCDCSTPCD
Screening of high production strain of β-glucosidase and its enzyme characteristics
从腐败的纤维质中分离筛选得到10株β-葡萄糖苷酶产生菌,与实验室保存的9株菌株一起做产酶发酵,其中B2酶活性最高,为24.4 U.mL－1,经菌种鉴定为黑曲霉,其β-葡萄糖苷酶最适合的pH值为4.5,最适温度为65℃,在pH值3.0～10.0较稳定；温度稳定性较好,在65℃保温120 min后仍有61.6％的相对活性；终浓度为5 mmol·L-1的Fe2+和Mn2+对酶活性有较强的促进作用.该酶较好的热稳定性和pH值稳定性使其显示出较好的应用潜力.
Ten β - glucosidase producing strains were isolated from the cellulosic samples. The highest production strain B2 was finally obtained by screening these 10 strains along with the other 9 strains identified previously as β-glucosidase producers. The B2, identified as Aspergillm niger, showed the yield of β-glucosidase of 24.4 U · mL-1 under certain conditions. The optimal pH-value of β -glucosidase was 4. 5 and the optimal temperature was 65 ℃. T…查看全部>>
岳冬冬;刘新育;徐新慧;王明道;王彩澜;陈红歌
河南农业大学生命科学学院,河南郑州450002河南农业大学生命科学学院,河南郑州450002河南农业大学生命科学学院,河南郑州450002河南农业大学生命科学学院,河南郑州450002河南农业大学生命科学学院,河南郑州450002河南农业大学生命科学学院,河南郑州450002
轻工纺织
β-葡萄糖苷酶黑曲霉筛选
β - glucosidase aspergillus niger screening
《河南农业大学学报》 2012 (2)
173-176,181,5
河南省科技攻关项目(2010A180006)
评论