化工进展2012，Vol.31Issue(5)：1096-1102,1108,8.
阿魏酸酯酶和木聚糖酶协同降解麦糟
Utilization of feruloyl esterase and xylanase for the degradation of brewers＇ spent grain
摘要
Abstract
The optimal fermentation time ofPenicillium citrinum feruloyl esterase （ PcFAE ） was 60 h and its activity was 40.8 mU/mL when the Brewers＇ Spent Grain （ BSG ） was used as the sole carbon source for Penicillium citrinum. One gram of alcohol-insoluble residue （ AIR ） of Brewers＇spent grain （ BSG ） was processed by the solution of crude xylanase （ XG180 ） （ 47.5 U/mL, 15 mL ） for 12 hours at the condition of the material-liquid ratio 1 ： 30（ W/V ）, pH 5.0 and 45 ℃, respectively. And then the solution of crude PcFAE （ 40.8 mU/mL ）was added and the sample was processed for another 12 h. The maximum release rate of Ferulic acid （ FA ） was 54.1% and the release amount of xylooligosaccharide （XOS） was 161 mg/g （BSG-AIR）, respectively. The results of our experiments showed that the release rate of FA and XOS from BSG-AIR increased significantly as the PcFAE could coordinate with the xylanase and enzymolysis process was also conducive to the degradation of BSG.关键词
阿魏酸酯酶/木聚糖酶/麦糟/生物降解Key words
feruloyl esterases/xylanase/brewers' spent grain/biodegradation分类
化学化工引用本文复制引用
李夏兰,程珊影,杨道秀,方柏山..阿魏酸酯酶和木聚糖酶协同降解麦糟[J].化工进展,2012,31(5):1096-1102,1108,8.基金项目
华侨大学人才启动基金（11BS221）及福建省科技重点项目（2011N0020）. （）
