化工学报2012，Vol.63Issue(5)：1522-1528,7.DOI:10.3969/j.issn.0438-1157.2012.05.029
非水相体系中海藻酸钠-壳聚糖微胶囊粒径及强度性能
Size and strength of alginate-chitosan microcapsules in nonaqueous system
摘要
Abstract
Immobilization technology is of potential in industrial application for improving activity and stability of biocatalyst in nonaqueous systems. Alginate-chitosan (AC) microcapsules as immobilization carrier were prepared by emulsification-internal gelation and complexation reaction, and five solvents were selected to form culture medium-solvent two pbase systems. At simulating yeast culture condition, AC microcapsules were put into the two phase systems and oscillated for 48 h. The morphology of AC microcapsules had no change for different two phase systems. For culture medium-dibutyl sebacate system, molecular weight of chitosan and formation time of microcapsule membrane were varied in a range of 40000-100000 and of 2-5 min, respectively, AC microcapsules with stable size, low broken rate and high mechanical strength were produced at conditions of low Mw (40000) chitosan and long formation time (5 min). These results showed that AC microcapsules prepared with above condition could be applied as immobilization carrier of biocatalyst. Moreover, yeast cells with biotransformation ability was used as a model of biocatalyst and entrapped in AC microcapsules. The growth of yeast cells entrapped in AC microcapsules to produce 2-phenylethanol was investigated in the culture medium-solvent two phase systems. The results showed that entrapped cells still keep its activity and can normal grow, which means that two phase systems have no adverse effect on it. Therefore! the immobilized yeast cells are of high biotransformation ability in the culture medium-solvent two phase systems with substrate concentration of 8-16 g· L-1.关键词
非水相/AC微胶囊/粒径/强度Key words
nonaqueous system/ AC microcapsule/ size/ strength分类
生物科学引用本文复制引用
侯丹丹,李会静,宋慧一,戴小敏,于炜婷,刘袖洞,马小军..非水相体系中海藻酸钠-壳聚糖微胶囊粒径及强度性能[J].化工学报,2012,63(5):1522-1528,7.基金项目
国家重点基础研究发展计划项目(2007CB714305) （2007CB714305）
国家自然科学基金项目(20736006,20806080) （20736006,20806080）
大连市青年科技人才基金(2007J23JH036). （2007J23JH036）
