化工学报2012，Vol.63Issue(7)：2271-2278,8.DOI:10.3969/j.issn.0438-1157.2012.07.041
黑曲霉负载银纳米颗粒的制备及其抗菌性能
In-situ synthesis and antibacterial performance of silver nanoparticle immobilized on Aspergillus niger
摘要
Abstract
A facile and eco-friendly route was presented to in-situ synthesize and immobilize silver nanoparticles (AgNPs, 6.9-8.2 nm) using Aspergillus niger as both reducing agent and support through the non-enzymatic reduction mechanism. The well-dispersed AgNPs/mycelium composite exhibited excellent antimicrobial activity against E. coli, and the minimal inhibitory concentration (MIC) was 217-434 mg · L-1 (based on the mass of AgNPs/mycelium) or 8-20 mg · L-1 (based on the mass of AgNPs). Although increasing reaction temperature from 30℃ to 60℃ resulted in increase of silver loading, the mean size of the AgNPs became larger at the same time, leading to the decrease of antimicrobial activity. The change of pH value from 9. 5 to 11. 5 promoted the reaction rate and shortened the reaction time (from 144 h to 24 h) , whereas no significant effect was observed on the antimicrobial activity. Finally, the release of AgNPs and Ag+ from the AgNPs/mycelium composite was investigated. The results suggested that the prepared AgNPs were supported firmly on Aspergillus niger and the release of Ag+ was 1. 7-6. 8 mg per gram of AgNPs/mycelium. Furthermore, increasing reaction temperature or pH value led to the decrease of Ag+ release.关键词
银纳米颗粒/生物还原/非酶催化/黑曲霉菌/抗菌性能Key words
silver nanoparticles/ bioreduction/ non-enzymatic/ Aspergillus niger/ antibacterial performance分类
通用工业技术引用本文复制引用
张金丽,孙道华,詹国武,林丽芹,郑艳梅,景孝廉,黄加乐,李清彪..黑曲霉负载银纳米颗粒的制备及其抗菌性能[J].化工学报,2012,63(7):2271-2278,8.基金项目
国家自然科学基金重点项目(21036004)和面上项目(20976146). （21036004）
