环境工程学报2012，Vol.6Issue(7)：2333-2338,6.
处理畜禽废水的序批式生物膜反应器中的细菌多样性
Bacterial diversity in sequencing batch biofilm reactor for treating livestock and poultry breeding wastewaters
摘要
Abstract
A sequencing batch biofilm reactor （SBBR） was used to treat livestock and poultry breeding wastewaters （LPBWs）. An operational mode of alternant aeration and non-aeration and 8 h every period was a- dopted with room temperatures ranging from 9 to 18℃ and DO during aeration phase controlled around 2 mg/L. Nitrite accumulation occurred and the removal efficiencies were as high as （95.1±0.8） % and （87.2± 0.6） % for NH3-N and TN, respectively. To reveal the bacterial community structure and its dynamic change trend in the SBBR, bacterial diversity was analyzed using PCR-DGGE technique and phylogenetic trees were constructed. The results indicated that no obvious change of bacterial community richness happened, taking the biofilm sam- ples of the start-up phase and the seeding sludge into comparison. Alternation between aeration and non-aeration during the running phase helped to improve the diversity index of bacteria in the biofilm, however, suffered the change of operational modes and ammonia load rates, the diversity indexes of ammonia-oxidizing bacteria during the running phase were slightly lower than that during the start-up phase. Except for anaerobic ammonia oxida- tion bacteria, some kinds of proteobacterium with denitrification function and special ammonia-oxidizing bacteria were convinced to exist in the biofilm under the experimental conditions, demonstrating that simultaneous short- cut nitrification and denitrification were the main mechanisms for the nitrogen removal.关键词
SBBR/畜禽废水/细菌多样性/PCR-DGGE/系统发育树Key words
SBBR/livestock wastewater/bacterial diversity/PCR-DGGE/phylogenetic tree分类
资源环境引用本文复制引用
肖鸿,樊明灏,彭宏,李远伟..处理畜禽废水的序批式生物膜反应器中的细菌多样性[J].环境工程学报,2012,6(7):2333-2338,6.基金项目
四川省教育厅科研项目 （）
