HPLC - ELSD法测定胃泰咀嚼片中黄芪甲苷质量比OACSTPCD
Determination of astragaloside in Weitai chewable tablets by HPLC-ELSD
建立胃泰咀嚼片中黄芪甲苷的质量比测定方法.采用高效液相色谱-蒸发光检测器定量分析黄芪甲苷的质量比,使用Alltima - C18(5μm,4.6 mm×250 mm)色谱柱,流动相为甲醇-水(68∶32),流速1 mL/min;蒸发光散射检测器(ELSD)飘移管温度50℃,载气压力1.58 Bar,放大系数(Gain)为10;进样量为20 μL.表明黄芪甲苷在0.95～22.8 μg之间呈良好的线性关系,r=0.999 00;加样回收率为98.9…查看全部>>
To establish an method for the determination of astragaloside in Weitai chewable tablets. Conducted determination of astragaloside in this drug by HPLC - ELSD. Chromato-gram condition; selected Alltima-C18(5 μm, 4. 6 mm×250 mm) ; mobile phase:methanol -water(68:32) ,speed of flow was 1.0 mL/min;the ELSD conditions were as follows; pressure of compressed air 1. 58 bar, drift temperature 50℃ and gain 10. The injection volume was 20 μL. The linear range of astr…查看全部>>
赵威;李云涛;杨波;杨书良
哈尔滨商业大学药学院,哈尔滨150076哈药集团世一堂制药厂,哈尔滨150088哈尔滨商业大学药学院,哈尔滨150076哈尔滨商业大学药学院,哈尔滨150076
医药卫生
胃泰咀嚼片黄芪甲苷HPLC-ELSD
Weitai chewable tablets astragaloside HPLC-ELSD
《哈尔滨商业大学学报（自然科学版）》 2012 (3)
271-273,3
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