河南农业科学2012，Vol.41Issue(4)：82-85,4.
棉花枯萎病菌激活蛋白的提取及其对棉花种子发芽的影响
Extraction of Activator Protein of Fusarium oxysporum and Its Effect on Germination of Cotton Seeds
摘要
Abstract
In order to explore biological control technologies of cotton Fusarium wilt,the extraction method and activity of activator proteins from Fusarium oxysporum were studied prelimina-rily in this article. F. oxysporum was isolated from cotton tissues infected by Fusarium wilt. The best culture conditions,26 ℃ ,200 r/min and 72 h were obtained by culturing F. oxysporum at dif-ferent temperatures,speed and time. And the dry weight of mycelia was up to 3. 611 g under this condition. The extracted proteins exhibited a relatively centralized electrophoretic band with the molecular weight of about 51 kD. The cotton seeds of two varieties (Xinluzhong No. 7,Xinluzhong No. 32) were treated by the activator protein which was diluted 1 000 times, 1 500 times and 2 000 times,and sterile water was used as CK. The effect of the activator protein diluent on seed germi-nation was detected,and the results indicated that it could promote seed germination and radicle growth of the two cotton seeds, with.1 500-time diluent performing remarkable effects. For Xin-luzhong No, 7,the seed germination rate was 90%,the radicle length wap 6. 04 cm,and the fresh and dry weight of radicle was 85. 78 mg and 8. 89 mg. The results were 85% ,5. 12 cm,77. 41 mg and 8, 56 mg, respectively, for Xinluzhong No. 32. These indicated that the activator protein had efficient induction activity, which could provide theoretic basis for development of low-cost protein biological pesticides.关键词
棉花/枯萎病菌/激活蛋白/种子发芽/生物防治Key words
cotton/ Fusarium oxysporum / activator protein/ seed germination/ biological control分类
农业科技引用本文复制引用
滕立平,王兰,但红霞..棉花枯萎病菌激活蛋白的提取及其对棉花种子发芽的影响[J].河南农业科学,2012,41(4):82-85,4.基金项目
塔里木大学校长基金项目(TDZKSS08009) （TDZKSS08009）
