河南农业科学2012，Vol.41Issue(4)：135-138,4.
南阳牛成纤维细胞的体外培养及生物学特性研究
Culture and Biological Characteristics of Nanyang Bovine Fibroblasts
摘要
Abstract
The purpose of this study was to preprare transgenic donor cells for somatic cell nuclear transfer by establishing bovine fibroblasts, based on culturing Nanyang fibrocystic successfully and detecting its biological character. The results demonstrated that DMEM/F12 containing 15% FBS is suitable for primary cell cultivation. Meanwhile,Nanyang fibrocystic was detected regularly by growth curve. Cell activity difference between cell freezing and cell reviving is not significant. Cell karyotype is normal when subcultured for six passages. Flow cytometric test results showed that GO - Gl phase of F4 and F8 generation cell increased by 17% to 20%. Compared to the S phase,F4 and F8 generation cell decrease 1% to 4% than that of Fll generation cells. The results indicated that the fibroblast cells obtained may be competent for bovine transgenic cloning.关键词
成纤维细胞/体外培养/南阳牛/生物学特性Key words
fibrocyte/ in vitro culture/ Nanyang bovine/ biological characteristic分类
农业科技引用本文复制引用
楚秋霞,安森亚,施巧婷,辛晓玲,王二耀,魏成斌,徐照学..南阳牛成纤维细胞的体外培养及生物学特性研究[J].河南农业科学,2012,41(4):135-138,4.基金项目
国家肉牛产业技术体系项目(rncytx—38) （rncytx—38）
