河南农业科学2012，Vol.41Issue(4)：139-142,145,5.
抗猪FcγRⅡb多抗阻断猪繁殖与呼吸综合征病毒抗体依赖增强作用研究
Inhibition of Antibody-dependent Enhancement of PRRSV Infection by Anti-poFcγRⅡb Polyclonal Antibodies
摘要
Abstract
In order to elucidate the role of porcine FcyR II b in antibody-dependent enhancement (ADE) ,F(ab') 2 fragments were generated from the IgG of anti-PRRSV sera. 104 TCID50 of PRRSV BJ-4 virions were pre-incubated with the F(ab')2 fragment (80 jug/mL) or anti-PRRSV sera (neutralizing antibody titer 1/5. 9,1/128 diluted) ,and then the opsonised virions were added to either porcine alveolar macrophages (PAMs) or Marc-145 cells. Sub-neutralizing antibodies observably enhanced the PRRSV infection in PAMs. No enhancement was observed in the normal Marc-145 cells and the F(ab')2 fragment had no effect on any cell lines. PAM cells were incubated with anti-poFcyR H b polyclonal antibodies and were then infected with PRRSV in the presence of anti-PRRSV antibody. Anti-poFcyR H b antibody inhibited the enhancement of infection when cells were infected in the presence of anti-PRRSV antibody vindicating that poFcyR II b can mediate ADE of PRRSV infection. This research should expand our understanding of the mechanisms of pathogenesis of PRRSV.关键词
抗体依赖增强(ADE)/阻断/IgGFc受体/猪繁殖与呼吸综合征病毒(PRRSV)/感染Key words
antibody-dependent enhancement/ inhibition/ IgG Fc receptors (FcyRs) / porcine reproductive and respiratory syndrome virus/ infection分类
农业科技引用本文复制引用
蒋志政,张改平,刘明阳,乔松林,刘永晖,王蕊,万博,鲍登克,王爱萍..抗猪FcγRⅡb多抗阻断猪繁殖与呼吸综合征病毒抗体依赖增强作用研究[J].河南农业科学,2012,41(4):139-142,145,5.基金项目
国家自然科学基金重点项目(30730068) （30730068）
