核农学报2012，Vol.26Issue(3)：433-438,6.
高能混合粒子场辐照对花生胚小叶组织培养及植株再生的影响
EFFECTS OF MIXED HIGH ENERGY PARTICLE FIELD ON EMBRYONIC LEAFLET CULTURE AND PLANT REGENERATION OF PEANUT
摘要
Abstract
In order to study effects of mixed high energy particle field on embryonic leaflet culture and plant regeneration of peanut（Arachis hypogaea L.）,dry seeds of peanut cultivar Luhua 11 were irradiated with five doses（0,67,105,150 and 196Gy）,then the embryonic leaflets were separated and incubated on MSB5 induction media containing 10mg/L 2,4-D,and the subcultures were subsequently transferred to MSB5 media supplemented with 4mg/L BAP.The results showed that the survival rate of explants decreased significantly with the increase of irradiation dose,except for that treated by 67Gy.Somatic embryo induction rate also showed no significant difference between CK and 67Gy,while that of 105Gy and 150Gy were much lower than that of 67Gy.Nevertheless,there was no somatic embryo formation in 196Gy.The frequencies of plant regeneration reduced remarkably with the increase of irradiation doses,except for that treated by 105Gy and 150Gy.According to the rate of survival explants,somatic embryo formation and plant regeneration,105Gy was the optimum mutation dose.The regenerated plantlets were grafted and transplanted to the field,and they eventually set seeds.关键词
花生/混合粒子场辐照/离体诱变/体胚诱导/植株再生Key words
peanut(Arachis hypogaea L.)/mixed high energy particle field irradiation/in vitro mutagenesis/somatic embryo induction/plant regeneration分类
农业科技引用本文复制引用
于新玲,刘录祥,乔利仙,隋炯明,郭宝太,徐丽娟,王晶珊..高能混合粒子场辐照对花生胚小叶组织培养及植株再生的影响[J].核农学报,2012,26(3):433-438,6.基金项目
国家自然基金项目 （）
863课题 （）
山东省中青年科学家奖励基金 （）
