核农学报2012，Vol.26Issue(3)：466-470,5.
绿色产色链霉菌E-219菌落的形态分化
MORPHOLOGICAL DIFFERENTIATION OF Streptomyces viridochromogenes E-219 ON SOLID CULTURE
摘要
Abstract
The Streptomyces viridochromogenes E-219 was derived from Streptomyces viridochromogenes CGMCC4.1119 treated with 60Co γ-rays irradiation and protoplast fusion.With the help of fluorescent probes,fluorescence microscope and electron microscopy,the morphology and development of E-219 on solid surface culture were investigated in this study.The effect of agarslant culture time on the production of Avilamycin was also studied to provide theoretical basis for industrial fermentation of selecting the appropriate seed to culture on the agarslant culture medium.The results implied that the development of colonies of Streptomyces viridochromogenes accompanied the intermittent hyhae apoptosis,and the production of spores was from the active mycelium.The colonial morphology of strain E-219 was significantly different from the original strain CGMCC4h1119.There were variegated hyphae formation in the stage of spore germination and initial hyphae development（10h） with the live and dead segments alternated in a highly regular fashion within the same hypha.After the early single colony formation,the third phase was followed by profuse growth of the live segments derived from the variegated hypha,then the second apoptosis of the mycelia（48h） was occurred with another quick growth,and sporulation was occurred at 96h.Strain CGMCC4.1119 had spiral sporotrichial and round conidiophores with spike,whereas strain E-219 had linear sporotrichial,smooth and cylindrical conidiophore.The results of shake flask experiments indicated that the spores of E-219 had the highest activity when cultured on agarslant culture medium and incubated for 106h with the production of avilamycin up to 1200mg/L.关键词
绿色产色链霉菌/形态发育分化/斜面培养/荧光染色/种龄Key words
Streptomyces viridochromogenes/morphological differentiation/agarslant culture time/fluorescence staining分类
农业科技引用本文复制引用
梁新乐,朱静,金英燕..绿色产色链霉菌E-219菌落的形态分化[J].核农学报,2012,26(3):466-470,5.基金项目
浙江省科技厅专项 （）
