核农学报2012，Vol.26Issue(3)：511-516,6.
食品辐照标志物DiHT的人工抗原合成及鉴定
SYNTHESIS AND IDENTIFICATION OF THE ARTIFICIAL ANTIGEN FOR DiHT IN IRRADIATION FOOD
摘要
Abstract
In the present study,5,6-Dihydrothymidine Hapten（DiHT-COOH） was synthesized by using 5,6-Dihydrothymidine（DiHT） as raw material.DiHT-COOH was identified by thin layer chromatography and 1H-NMR.Hapten was conjugated to bovine serum albumin（BSA） and ovalbumin（OVA） to obtain artificial immune antigen and coating antigen,respectively.After detected by UV spectroscopy and SDS-PAGE,the ratios of Hapten to protein were calculated.The conjugation ratios of immunogen and coating antigen were 9.8∶1 and 12∶1,respectively.Antisera were obtained by immunizing BALB/c with immunogen and the titer were 1∶2.56×104,which indicated the synthesis of artificial antigen was successful.关键词
二氢脱氧胸腺嘧啶/半抗原/人工抗原/酶联免疫Key words
DiHT/Hapten/artificial antigen/ELISA分类
农业科技引用本文复制引用
王志伟,潘家荣,李淑荣,王志东,高美须,李秉超,睢珂,王磊..食品辐照标志物DiHT的人工抗原合成及鉴定[J].核农学报,2012,26(3):511-516,6.基金项目
国家科技支撑计划项目 （）
国防科技工业民用专项科研技术研究项目 （）
