红外与毫米波学报2012，Vol.31Issue(3)：248-253,6.DOI:10.3724/SP.J.1010.2012.00248
分子超光谱成像系统应用于糖网病药物疗效研究
Evaluation of erythropoietin efficacy on diabetic retinopathy based on molecular hyperspectral imaging (MHSI) system
摘要
Abstract
Integrate imaging technology, spectrum technology and microscope, a novel molecular hyperspectral imaging (MHSI) system based on AOTF (acousto-optic tunable filters) was presented. The system was used to capture hyperspectral images of rat retinal sections selected from three groups: normal(N), diabetic(D), and EPO-treated(EO). By processing hyperspectral images data of 30 samples, single band images and the pseudocolor images of N,D,EO group are gained. Typical spectra curves of the ONL cells of N,D,E0 are extracted. By analyzing these images, descending order of the ONL thickness is N,E0,D. The experimental results cite that the ONL thickness of E0 group will be increased after EPO treated. By analyzing these spectrums, transmission intensity of D group is generally higher than that of N group in the spectral field from 550 nm to lOOOnm and transmission intensity of E0 group is in the middle of D and N. By analyzing spectrum relations, results show the spectral signature of the E0 group was more similar to that of N than that of D to N. The above results suggest EPO may prevent cell apoptosis and has protective effect on rat DR at a certain level. MHSI system may probably provide a potential approach to researching pathogenesis ,causes of blindness and drug efficacy of DR in future.关键词
糖网病/分子超光谱成像系统/促红细胞生长素/外核层/光谱角Key words
Diabetic retinopathy/ MHSI/ EPO/ ONL/ Spectral Angle分类
数理科学引用本文复制引用
刘洪英,李庆利,胥亮,王依婷,刘锦高,薛永祺..分子超光谱成像系统应用于糖网病药物疗效研究[J].红外与毫米波学报,2012,31(3):248-253,6.基金项目
国家自然科学基金[量子点标记的肿瘤新生血管双模分子成像及识别方法研究(61177011) （61177011）
荧光磁性复合量子点可控制备及其在多摸成像中的应用研究(60976004) （60976004）
分子光谱成像的生物组织定量检测机理与方法研究(60807035)] （60807035）
