渔业科学进展2012,Vol.33Issue(2):43-48,6.
患诺卡氏菌病的大黄鱼几种主要组织的酶活力变化分析
The analysis of enzymatic activities in several major tissues of Pseudosciaena croceas infected with nocardiosis
摘要
Abstract
In order to reveal the effect of nocardiosis on Pseudosciaena croceas physiological conditions,the process of physiological and biochemical pathology was explored in this article.With the method of enzymology analysis,the change of enzyme activity in several main lesion tissues of P.croceas infected with Nocardia was tested.The results indicated that,in contrast with the control group,the amylase activities had no significant change in heart and spleen,while decreased apparently in kidney,liver and gill(P<0.01).Lysozyme activities in heart,liver,kidney and gill increased significantly(P<0.01),whereas declined significantly in spleen (P<0.01).Alkaline phosphatase activities in heart,spleen,liver and gill increased significantly,while became lower in kidney (P<0.01).Acidic phosphatase activities were higher in heart,spleen,and lower in kidney and liver,while no significant change in gill was found.Both glutamic-oxalacetic transaminease and glutamic-pyruvic transaminase increased significantly in kidney and decreased apparently in liver(P<0.01),while none of them showed significant change in heart,spleen and gill(P>0.05).Malondialdehyde both in kidney and liver decreased and had no significant changes in heart,spleen and gill.Superoxide dismutase activities reduced significantly in liver and gill(P<0.01),while no significant changes in heart,spleen.The cata-lase activities reduced significantly in heart and kidney,and increased in spleen(P<0.01),while no significant changes in liver or gill.Therefore,P.croceas had obvious stress response to Nocardia infection and showed different levels of damage to the organs of sicken fishes.关键词
大黄鱼/诺卡氏菌病/酶活力Key words
Pseudosciaena croceas/Nocardiosis/Enzyme activity分类
农业科技引用本文复制引用
安树伟,袁思平,王国良..患诺卡氏菌病的大黄鱼几种主要组织的酶活力变化分析[J].渔业科学进展,2012,33(2):43-48,6.基金项目
农业部公益性行业科研专项(200903029)、教育部长江学者与创新团队项目(IRT0734)和浙江省自然科学基金项目(Y304078)共同资助 (200903029)