渔业科学进展2012，Vol.33Issue(2)：97-103,7.
5种食源性致病菌多重PCR检测方法的建立
Multiplex PCR for the detection of five major foodborne pathogens
摘要
Abstract
A multiplex polymerase chain reaction was developed for the simultaneous detection of five major foodborne pathogens,Vibrio parakaemolyticus,Staphylococcus aureus,Listeria monocy to genes,Salmonella enteritidi,and Shigella flexneri.Five pairs of specific primers were designed according to the thermolabile hemolysin gene of V.parahaemolyticus,the heat stable nuclease gene of S.aureus,the Listeriolysin O gene of L.monocy to genes,the invasion protein A gene of S.enteritidis,the invasion plasmid antigen H gene of S.flexneri.The reaction conditions were optimized,and the specificity and sensitivity of this method were tested.The detecting results of 48 samples also proved its reliability and practicability.关键词
多重PCR/食源性病原菌/检测Key words
Multiplex PCR/Food borne pathogens/Detection分类
农业科技引用本文复制引用
程晓艳,刘庆慧,黄倢..5种食源性致病菌多重PCR检测方法的建立[J].渔业科学进展,2012,33(2):97-103,7.基金项目
国家自然科学基金(30871942)和对虾行业专项(201103034)课题共同资助 （30871942）
