家畜生态学报2012，Vol.33Issue(3)：23-27,5.
两种断乳方式对小鼠乳腺中β-防御素1 mRNA表达的影响
Effect of Delactation Methods on the Expression of β-defensin 1 mRNA of Mammary Gland in Mice
摘要
Abstract
β-defensin has a broad-spectrum microbicidal activity and is an important regulator of innate immunity.The aim of this study was to examine the express of β-defensin l(Dcfbl) after forced weaning and natural weaning.72 female mice were divided into forced weaning group (n=6) and natural weaning group (n = 6) after parturition.At the day of weaning (0 d) and within 5 days after weaning (1 d, 2 d, 3 d, 4 d and 5 d) , the mice were euthanatized.The forth mammary gland tissue were taken and examined with semi-quantitative RT-PCR.The results showed that the expression of Defbl mRNA significantly decreased (P<C0.05)on 4 d compared with the day of weaning(0 d) in forced weaning group.In natural weaning group, the expression of (3-defensinl mRNA significantly decreased at 1 d, 2 d and increased sharply (P<0.05)at 3 d compared with the day of weaning(0 d).关键词
β-争防御素1/自然断乳/强制断乳/小鼠Key words
β-defensinl/ natural delactation/ forced delactation/ mice分类
农业科技引用本文复制引用
孟丽丽,朱河水,钟凯,王月影..两种断乳方式对小鼠乳腺中β-防御素1 mRNA表达的影响[J].家畜生态学报,2012,33(3):23-27,5.基金项目
河南省教育厅自然科学研究资助计划项目(2010A230010) （2010A230010）
