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一株芽孢杆菌的分离鉴定及其益生潜质分析

徐海燕 曹斌 辛国芹 曹银生 武香玉 陈静 谢全喜 谷巍

家畜生态学报2012,Vol.33Issue(3):48-54,7.
家畜生态学报2012,Vol.33Issue(3):48-54,7.

一株芽孢杆菌的分离鉴定及其益生潜质分析

Isolation and Identification of a Bacillus Subtilis Strain and its Potential Probiotic Analysis

徐海燕 1曹斌 1辛国芹 1曹银生 1武香玉 1陈静 1谢全喜 1谷巍1

作者信息

  • 1. 山东宝来利来生物工程股份有限公司,山东泰安271000
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摘要

Abstract

A Bacillus strain BF3 ,which was isolated from the feces of healthy and adult broiler, was i-dentified as Bacillus subtilis by its cultural characteristics,morphological characteristics,physiological and biochemical properties and 16S rDNA sequence.Inhibition of the growth of enteric bacteria by Bacillus subtilis BF3 was studied.The results showed that the Bacillus subtilis BF3 could decrease the propagation of the Salmonella pullorum and Chicken Escherichia coli obviously in the course of incubation.The strength of the antagonism was influenced by the inoculation time of Bacillus and pathogenic.The Bacillus strain was inoculated firstly,the antagonism was the strongest.Bacillus and pathogenic bacteria were inoculated at the same time, the antagonism ranked the second.The Enteric bacterium was inoculated firstly,the antagonism was the weakest.And the Bacillus strain BF3 metabolites showed powerful ability to inhibit the growth of the Salmonella pullorum and Chicken Escherichia coli.

关键词

分离鉴定/枯草芽孢杆菌/肠道致病菌/拮抗

Key words

isolation and identification/Bacillus subtilis /enteric bacterium/inhibition

分类

农业科技

引用本文复制引用

徐海燕,曹斌,辛国芹,曹银生,武香玉,陈静,谢全喜,谷巍..一株芽孢杆菌的分离鉴定及其益生潜质分析[J].家畜生态学报,2012,33(3):48-54,7.

家畜生态学报

OA北大核心CSTPCD

1673-1182

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