基础医学与临床2012，Vol.32Issue(6)：608-612,5.
新型HER2适配体的筛选及鉴定
Identification of a novel DNA aptamer against HER2
摘要
Abstract
Objective To develop HER2 DNA aptamers that may potentially serve as a tumor-binding ligand in novel HER2-targeted therapeutic strategies. Methods A single-stranded 86nt DNA library containing 40 random oli-gonucleotides was synthesized in vitro. A new aptamer HA5 was developed by SELEX technique with HER2 epitope peptide as target. Flow cytometry was performed to monitor the enrichment of aptamer pool and to evaluate the binding characterization of HA5. The structure of HA5 was predicted by MFold. Results The selected HA5 bound to the HER2 and had minimal cross reactivity to trypsin. In addition, the aptamer was found to preferentially bind to HER2-positive but not to HER2-negative cells. Conclusions HA5 may have application potentials in targeted therapy against HER2-positive malignancies.关键词
适配体/SELEX/HER2/乳腺癌Key words
aptamer/ SELEX/ HER2/ breast cancer分类
医药卫生引用本文复制引用
刘喆,杨先达..新型HER2适配体的筛选及鉴定[J].基础医学与临床,2012,32(6):608-612,5.基金项目
国家重大科学计划(2011CB933504,2011CB911003,2011CB911004) （2011CB933504,2011CB911003,2011CB911004）
国家自然科学基金(81071870) （81071870）
