基础医学与临床2012，Vol.32Issue(6)：628-633,6.
肝内及肝外胆管癌表达下调microRNAs表达谱的鉴定
Identification of down-regulated miRNAs in extrahepatic and intrahepatic cholangiocarcinoma
摘要
Abstract
Objective To investigate the expression profile of miRNAs down-regualted in human extrahepatic and intrahepatic cholangiocarcinoma tissues and to probe the effect on cell growth of four of these miRNAs in QBC939 cell line. Methods Down-regulated miRNAs in extrahepatic or intrahepatic cholangiocarcinoma (issues were analyzed by miRNA-microarray and confirmed by miRNA real-time PCR analysis. Based on these findings, four of these down-regulated miNRAs were chosen to perform function investigation. The miRNAs mimics were transfect-ed into QBC939 cells and cell proliferation assay was performed by using MTT, which would demonstrate the relationship between down-regulated miRNAs and eholangiocarcinoma cell growth. Results 25 miRNAs and 15 miRNAs were down-regulated in extrahepatic cholangiocarcinoma and intrahepatic cholangiocarcinoma, respectively. There were 6 miRNAs down-regulated both in two types of cholangiocarcinoma. miR-181a, miR-596, miR-492, and miR - 6 0 2 were overexpressed in QBC 9 3 9 cells, which indicated a significant inhibitory effect on cell growth.Conclusions The miRNAs expression patterns in human extrahepatic and intrahepatic cholangiocarcinoma tissues are different and down-regulated miRNAs act as suppressors on cholangioearcinoma cell growth.关键词
microRNA/胆管癌/细胞增殖Key words
cholangiocarcinoma/ microRNAs/ cell growth分类
医药卫生引用本文复制引用
刘长征,刘卫,于岚,蔡磊,李静静,何小东,陈松森..肝内及肝外胆管癌表达下调microRNAs表达谱的鉴定[J].基础医学与临床,2012,32(6):628-633,6.基金项目
国家自然科学基金青年科学基金(81100608) （81100608）
