基础医学与临床2012，Vol.32Issue(6)：639-643,5.
体外三维培养骨髓间充质干细胞诱导分化为肝细胞及其极化特征
Differentiated hepatocytes derived from human bone mesenchymal stem cell in 3-dimentional culture and the polarity characteristic
摘要
Abstract
Objective To explore hepatic differentiation of human bone mesenchymal stem cell (BMSC) in 3-dimentional (3D) culture system of rat type-I collage and the polarity of differentiated hepatoeyte. Methods BMSCs were isolated from adult bone marrow and differentiated to hepatocyte in 3D culture system of rat type-I collage. The morphological and structural characteristics of differentiated hepatocyte derived from BMSC in 3D culture were mi-croscopied HE staining. The expression of hepatocyte-specific markers of ALB and AFP was analyzed using immu-nohistochemical staining. The expression of hepatoeyte polarity protein BSEP and SRB1 was assessed using the Im-munofluorescence. Results Differentiated hepatoeyte derived from BMSC formed tight junction and tubular or spherical structures in 3D culture system. The expression of ALB and AFP in hepatocytes differentiated from BMSC was positive. The expression of BSEP and SRBI in the 3D culture was positive and characteristic. Conclusions BMSC can differentiate to hepatoeyte in 3D culture. The differentiated hepatoeyte can form hepatocyte-specific polarity in 3D culture.关键词
骨髓间充质干细胞/三维培养/肝细胞/极化/分化Key words
bone mesenchymal stem cell/ 3-dimentional culture/ hepatoeyte/ polarity/ differentiation分类
医药卫生引用本文复制引用
卫金花,曲强,杨阳,何小东,刘卫..体外三维培养骨髓间充质干细胞诱导分化为肝细胞及其极化特征[J].基础医学与临床,2012,32(6):639-643,5.基金项目
国家自然科学基金(30871427) （30871427）
