江汉大学学报：自然科学版2012，Vol.40Issue(3)：77-82,6.
cAMP依赖蛋白激酶（PKA）磷酸化Nef蛋白对HIV复制的影响
Impact of HIV Replication by PKA Phosphorylation of HIV Nef
李培林1
作者信息
- 1. 哈佛大学医学院医学系,波士顿MA02115 加州大学旧金山分校医学院医学系,旧金山CA94121
- 折叠
摘要
Abstract
Objective： To study impact of Nef PKA phosphorylation site on HIV replication. Methods： To mutate Nef Ser9 to alanine in the context of full-length HIVNL4-3 to make a single mutant of HIV DNA, then make viral stock by using either wild-type HIV or the single mutant HIV. Finally using these viral stocks to infect PBMCs. Results： Virus made from single mutant of Ser9 to alanine lowered HIV replication in resting PBMCs compared to parental virus. Conclusion： This mutation plays a major role in abrogating the Nef effect on HIV replication in unstimulated primary cells. This result suggests that Nef phosphorylation by PKA is an important step in the viral life cycle in resting cells.关键词
HIV/Nef蛋白/磷酸化/蛋白激酶PKAKey words
HIV/Nef/phosphorylation/protein kinase A分类
医药卫生引用本文复制引用
李培林..cAMP依赖蛋白激酶（PKA）磷酸化Nef蛋白对HIV复制的影响[J].江汉大学学报：自然科学版,2012,40(3):77-82,6.
