经济动物学报2012，Vol.16Issue(2)：76-78,3.
珍珠鸡源IBDV的分离鉴定及其卵黄抗体的制备
Isolation and Identification of Infectious Bursal Disease Virus from Guinea Fowl and Preparation of Egg Yolk Immunoglobulin
摘要
Abstract
A field virus isolated from guinea fowl was identified to be an infectious bursal disease virus（IBDV） by chicken embryo inoculation test,agar-gel diffusion test,PCR rapid detection test and virus gene sequencing.The egg laying chicken were immunized with inactivated oil emulsion vaccine developed with the infectious bursal disease virus isolated from guinea fowl to prepare egg yolk immunoglobulin.The result showed that the egg yolk immunoglobulin titer of IBDV determined by APG was 1∶256 and no bacteria were found in the egg yolk immunoglobulin and no any obvious adverse reaction to the chicken.The clinical trial showed that the effective rate of treatment was 91% and effective rate of prevention was 100% for the chicken.关键词
珍珠鸡/鸡传染性法氏囊病病毒/分离鉴定/卵黄抗体Key words
guinea fowl/IBDV/isolation and identification/egg yolk immunoglobulin分类
农业科技引用本文复制引用
徐亚萍,曾祥伟,李志军,李梦溪..珍珠鸡源IBDV的分离鉴定及其卵黄抗体的制备[J].经济动物学报,2012,16(2):76-78,3.基金项目
东北林业大学国家级大学生创新性实验计划项目 （）
中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室开放基金项目 （）
