军事医学2012，Vol.36Issue(8)：630-633,4.
核酸适体和胶体金检测皮质醇含量的方法与应用
Cortisol tested based on aptamers and colloidal gold
摘要
Abstract
Objective To obtain and identify aptamers which can specifically bind to cortisol by systematic evolution of ligands by exponential enrichment ( SELEX ) protocol, and to establish a sensitive method of quantitative detection of cortisol levels by using aptamers and colloidal gold. Methods Colloidal gold was prepared by sodium citrate reduction and sodium borohydride reduction method. Cortisol levels were measured using the aptamer and colloidal gold aggregation chromo-genic method. Results Compared to 20 nm colloidal gold particles, five and 13 run colloidal gold particles were of good sensitivity, reproducibility and linear range. Using 5 nm colloidal gold particles, the linear range of detection of cortisol was 10. 1 -320 μmol /L, the linear correlation coefficient r =0.9993. The standard deviation was less than 10% of the average with good reproducibility. This method is currently suitable for the detection of cortisol standard. Conclusion This experiment establishes a highly sensitive specific detection method of cortisol.关键词
SELEX适体技术/皮质醇/核酸适体/胶体金/检测Key words
SELEX aptamer technique/ cortisol/aptamer/gold colloid/test分类
医药卫生引用本文复制引用
张岭,弓景波,王瑜,石卿,刘伟丽,梅竹松,钱令嘉,姚林..核酸适体和胶体金检测皮质醇含量的方法与应用[J].军事医学,2012,36(8):630-633,4.基金项目
国家科技重大专项课题(2008ZXJ090-011) （2008ZXJ090-011）
国家自然科学基金青年资助项目(81000584) （81000584）
