江西农业学报2012,Vol.24Issue(6):5-8,4.
不同植物查尔酮合成酶CHS基因的生物信息学分析
Bioinformatics Analysis of Chalcone Synzyme (CHS) Genes in Different Plants
摘要
Abstract
In this paper, the nucleic acid sequences and the conjectural amino acid sequences of chalcone synthase (CHS) genes registered in GenBank in 10 plant species were analyzed by using bioinformattcs method. Several parameters of these sequences, including physicochemical property, signal peptide, transmernbrane regions, hydrophobicity or hydrophilicity, functional domains and secondary structures, were detailedly analyzed, and the phylogenetic tree was constructed for CHS amino acid sequences. The results showed that the full - length of ORF of 10 CHS genes was about 1.2 lcb and encoded 399 amino acids. All amino acid sequences of CHS in different plant species contained 1 potential N - glycosylation site (32NMSS), 4 protein kinase c phosphorylation sites (69TIR, 158SVK, 202TFR, 359SAK) and 1 chalcone synthase active position (161RLMMYQQGCFAGGTVLR). All the amino acid sequences were hydrophobicity protein, without signal peptide and transmernbrane regions.关键词
查尔酮合成酶/生物信息学/功能分析/结构预测Key words
Chalcone synzyme/ Bioinformatics/ Function analysis/ Structure prediction分类
生物科学引用本文复制引用
张弢..不同植物查尔酮合成酶CHS基因的生物信息学分析[J].江西农业学报,2012,24(6):5-8,4.基金项目
青岛农业大学校高层次人才启动基金(630745). (630745)