军医进修学院学报2012，Vol.33Issue(9)：953-956,4.DOI:10.3969/j.issn.1005-1139.2012.09.021
间充质干细胞移植早期对心肌细胞凋亡及左心室功能的影响
Effect of early transplanted mesenchymal stem cells on apoptosis of myocardial cells and function of left ventricle
摘要
Abstract
Objective To study the effect of early transplanted bone marrow-derived mesenchymal stem cells (MSC) on apoptosis of myocardial cells, hemodynamics and function of left ventricle (LV) in rats following acute myocardial infarction (AMI). Methods MSC were isolated from Wistar rats by density gradient centrifugation and incubated with adherent culture method. A rat AMI model was established by permanent ligation of the left anterior descending coronary artery. Thirty minutes later, the animals were randomly divided into MSC group (n=10), AMI group (n=10), and sham operation group (n=5). Apoptosis of myocardial cells, hemodynamics and function of LV were monitored by echocardiography and TUNEL 72h after transplantation of MSC. Results The mean arterial blood pressure, left ventricular systolic pressure, left ventricular ejection fraction, and shortened fraction were significantly lower while the left ventricular end diastolic pressure was significantly higher in AMI group than in sham operation group. The rate of cardiac infarction and apoptosis of myocardial cells was 41.6% and 18.9%, respectively, which decreased to 32% and 51%, respectively, after MSC transplantation (P<0.01). However, no significant difference was found in the mean arterial blood pressure, LV systolic pressure, end LV diastolic pressure and LV function between the two groups (P>0.05). Conclusion Early MSC transplantation decreases the apoptosis of myocardial cells but cannot improve the left ventricular function of rats following AMI.关键词
骨髓间充质干细胞/细胞移植/急性心肌梗死/凋亡Key words
bone marrow mesenchymal stem cell/ cell transplantation/ acute myocardial infarction/ apoptosis分类
临床医学引用本文复制引用
文毅,边素艳,王红叶..间充质干细胞移植早期对心肌细胞凋亡及左心室功能的影响[J].军医进修学院学报,2012,33(9):953-956,4.基金项目
国家自然科学青年基金(81100107) （81100107）
解放军总医院科技创新基金(10KMM22) （10KMM22）
