昆明医学院学报2012，Vol.33Issue(6)：28-31,4.
抗整合素αⅤβ3单抗抑制上皮性卵巢癌血管及肿瘤生成的实验研究
The Experiment Research on the Inhibiting Effect of Angiogenesis and Tumor in EOC by Integrin αⅤβ3 Antibody
摘要
Abstract
Objective To explore the apoptosis induction effect of vascular endothelial cells and the inhibiting effect of angiogenesis and tumor growth in EOC by anti-integrin a V β 3 antibody. Methods The EOC TYK cells were implanted into CAM to establish the EOC CAM model, Anti- integrin a V β 3 antibody (experiment group) and NS (control group) were added respectively into CAM at 12h after cells implanted. N , A, VA/A and T of CAM in two groups were determined by image analysis on 5 d. HUVEC ECV304 cells were implanted on the Vn surface, the cell apoptosis rate were counted at 0 h, 12 h, 24 h, 48 h and 72 h. Results The N, A, VA/A and T were 16.80 ±0.71, 10.02 ±4.48 mm2, 25.50 ± 11.41 and 8.25 ±4.15 mm2 respectively in experiment group, which were significantly lower than those in control group, which were 42.20 ± 15.32, 26.15 ± 8.35 mm2, 66.62 ± 17.64 and 34.26 ± 15.83 mm2 respectively (P < 0.05). The HUVEC were induced to apoptosis by integrin a V β 3 antibody in a time dependent manner, the apoptosis rate at 24 h, 48 h and 72 h in integrin a V β 3 antibody group were significantly higher than those in IgG group. Conclusion Integrin a V β 3 antibody inhibits angiogenesis and tumor growth in EOC by inducing vascular endothelial cells apoptosis, which might be a treatment target of EOC.关键词
整合素αⅤβ3/抗体/EOC/血管生成Key words
Integrin a V β 3/ Antibody/ EOC/ Angiogenesis分类
医药卫生引用本文复制引用
杨丽华,周轶平,苏莹,胡万芹..抗整合素αⅤβ3单抗抑制上皮性卵巢癌血管及肿瘤生成的实验研究[J].昆明医学院学报,2012,33(6):28-31,4.基金项目
云南省科技厅自然科学研究基金资助项目(2009ZC113M ) （2009ZC113M ）
