兰州大学学报（医学版）2012，Vol.38Issue(2)：1-3,9,4.
胃癌hMLH1基因启动子CpG岛甲基化
Hypermethylation of CpG island at the hMLH1 gene promoter in gastric carcinomas
摘要
Abstract
Objective To determine the Hypermethylation of CpG island at the hMLHl gene promoter by utilizing methylation-specific polymerase chain reaction analysis. Methods The methylation stata of the hMLHl promoter CpG-island were investigated in 2 human gastric cancer cell lines, 31 primary human gastric carcinomas, and 23 adjacent gastric tissues. Results CpG-island hypermethylaion was not detected in control biopsy specimens but identified in human gastric cancer cell lines and 11 of gastric carcinoma patients. CpG-island hypermethylaion of hMLHl gene was detected in 7 adjacent gastric tissues. In primary gastric tumors, a significant association was not found between tumor differentiation status and lymph node metastasis and CpG island methylation status of hMLHl gene. Conclusion Hypermethylation of CpG island at the hMLHl gene promoter is frequently detected in gastric carcinoma. hMLHl promoter hypermethylation may involve in the early gastric carcinogeneais process.关键词
hMLH1/胃癌/CpG岛/甲基化Key words
hMLHl/ gastric carcinoma/ CpG island/ hypermethylation分类
医药卫生引用本文复制引用
姬瑞,郭娴,郭庆红,陈兆峰,李强,周永宁,杨挚..胃癌hMLH1基因启动子CpG岛甲基化[J].兰州大学学报（医学版）,2012,38(2):1-3,9,4.基金项目
兰州大学中央高校基本科研业务费专项资金(lzujbky-2009-149) （lzujbky-2009-149）
