临床肝胆病杂志2012，Vol.28Issue(6)：435-438,4.
热休克蛋白90抑制剂17- AAG对乙型肝炎病毒复制的抑制作用
17- AAG, an inhibitor of heat shock protein 90, down-regulates hepatitis B virus replication
摘要
Abstract
Objective To investigate the potential involvement of the heat shock protein 90 ( HSP90) in transforming growth factor - beta (TGFfJ) signaling activation in hepatocytes, and to observe the effect of the HSP90 inhibitor, 17 -AAG, on hepatitis B virus (HBV) replication. Methods HepG2 cells were treated with 17 - AAG and total RNA was extracted to measure PAI - 1 (SERPINE1) expression by real - rime RT - PCR. Meanwhile, HepG2 cells were also transfected with the HBV1.3 HBV replicative plasmid after two hours of pretreat-ment with 17 -AAG and TGFfJ or SB431542. At day4 post -transfection, HBV core particles were extracted and HBV replicative intermediates were detected by Southern blotting analysis. HBV surface antigen (HBsAg) was measured by EL1SA. Results 17 - AAG down -regulated mRNA expression of PAI -1 in HepG2 cells. HBV replication and HBsAg expression were inhibited upon 17 - AAG treatment. However, activation of the TGFβ signaling pathway had no effect on HBV replication. Conclusion HSP90 is involved in the TGFfJ signaling pathway during HBV infection. The HSP90 inhibitor, 17 - AAG, can inhibit HBV replication and HBsAg expression through a TGFfJ -independent signaling mechanism.关键词
HSP90热休克蛋白类/肝炎病毒,乙型/转化生长因子βKey words
HSP90 heat - shock - proteins/ hepatitis B virus/ transforming growth factor beta分类
医药卫生引用本文复制引用
柯晓煜,黄红平,王秋景,余源,刘慎沛,张春燕,陈妍,杨燕,杨东亮..热休克蛋白90抑制剂17- AAG对乙型肝炎病毒复制的抑制作用[J].临床肝胆病杂志,2012,28(6):435-438,4.基金项目
国家自然科学基金(30700701) （30700701）
中央高校基本科研业务费专项资金(2011JC061) （2011JC061）
