临床神经病学杂志2012，Vol.25Issue(2)：115-118,4.
中药补阳还五汤对脑出血大鼠脑组织水通道蛋白4表达的影响
Effects of Buyang Huanwu decoction on expression of Aquaporin-4 in brain tissue of intracerebral hemorrhage rats
摘要
Abstract
Objective To explore the effects of Buyang Huanwu decoction on expression of Aquaporin-4 (AQP4) in brain tissue of intracerebral hemorrhage (ICH) rats. Methods Fifty-five male SD rats were randomly divided into sham operation group ( n = 5 ) , ICH models group ( n = 25) and Buyang Huanwu decoction therapy group (n = 25). The ICH model rats were made ICH in right striatum by autoblood injection method. The sham operation group was just received the same operation without injecting its autoblood. The Buyang Huanwu decoction therapy group was gavaged with 1 ml/100 g Buyang Huanwu decoction for 3 d before operation. The sham operation group and the ICH models group were gavaged with normal saline instead during the same period. The neurological function deficit scale was scored at the relevant time and the rats were sacrificed. The expression of AQP4 protein in brain tissue was detected by immunohistochemistry, and the expression of AQP4 mRNA was detected by hybridization in situ method. Results The neurological deficit scores of Buyang Huanwu decoction therapy group and ICH models group were obviously higher than that of sham operation group (all P < 0. 05 ). The neurological deficit scores of Buyang Huanwu decoction therapy group at 3 d and 7 d after operation were obviously lower than that of ICH models group (all P < 0.05). The expression of AQP4 protein and mRNA in brain tissue of Buyang Huanwu decoction therapy group and ICH models group were obviously higher than that of sham operation group (all P<0. 05). The expression of AQP4 protein and mRNA in brain tissue of Buyang Huanwu decoction therapy group at 3 d and 7 d after operation were obviously lower than that of sham operation group (all P < 0. 05). There was no obvious adverse reaction of all the rats in all the groups. Conclusions Buyang Huanwu decoction can inhibit the expression of AQP4 in brain tissue of ICH rats, and is helpful to progress the neurological function. The effective of Buyang Huanwu decoction therapy maybe related with its inhibition of AQP4 and alleviate of brain edema.关键词
补阳还五汤/脑出血/水通道蛋白4Key words
Buyang Huanwu decoction/ intracerebral hemorrhage/ Aquaporin-4分类
医药卫生引用本文复制引用
孙皓,郭富强,王多姿,张红艳,汪瑾宇..中药补阳还五汤对脑出血大鼠脑组织水通道蛋白4表达的影响[J].临床神经病学杂志,2012,25(2):115-118,4.基金项目
四川省卫生厅科研基金项目(020019) （020019）
四川省中医药管理局科研基金项目(200669) （200669）
