临床神经病学杂志2012，Vol.25Issue(3)：196-198,3.
依达拉奉预处理对小鼠脑缺血再灌注损伤后皮质一氧化氮合酶表达的影响
Effect of Edaravone pretreatment on the expression of nitric oxide synthase in cortex of mice after cerebral ischemia reperfusion injure
摘要
Abstract
Objective To explore the effect of Edaravone pretreatmenl on the expression of nitric oxide synthase ( NOS) in cortex of mice after cerebral ischemia reperfusion (IR) injure. Methods Forty-eighi male ICR mice were rendomly divided into sham-operation group, control group and Edaravone group. Edaravone 3 mg/(kg·d) and same volume of normal saline were intraperitoneally injected in Edaravone group and control group respectively for 7 d. Then, the IR models were established. After f h ischemia and 24 h reperfusion, the volume of infarct was measured by 2, 3, 5-chlorinated three phenyl four nitrogen (TTC) dyeing method. The positive cells of neuronal, inducible and endothnlial NOS ( nNOS, iNOS, eNOS) in cortex were detected by immunohistochemistry. Results Compared with the sham-operation group, the quantity of nNOS, iNOS and eNOS positive cells in cortex in control group were significantly increased ( all P < 0. 05 ) . Compared with the contral group, the infarct volume in Edaravone group was significantly smaller, the quantity of nNOS and iNOS positive cells in cortex were significantly decreased, and the quantity of eNOS positive cells was significantly increased (all P < 0. 05 ). Conclusion Edaravone pretreatment has the neuroproteetion through to change the expression of nNOS, iNOS and eNOS in cortex of IR mice.关键词
脑缺血再灌注/一氧化氮合酶/依达拉奉Key words
ischemia reperfusion/ nitric oxide synthase/ Edaravone分类
医药卫生引用本文复制引用
李健,朱沂,党辉,沙晶,艾山江,景燕,李红燕,玛依努尔,陆明佳,补娟..依达拉奉预处理对小鼠脑缺血再灌注损伤后皮质一氧化氮合酶表达的影响[J].临床神经病学杂志,2012,25(3):196-198,3.基金项目
新疆维吾尔自治区自然科学基金(2010211A56) （2010211A56）
