临床神经外科杂志2012，Vol.9Issue(3)：133-135,3.DOI:10.3969/j.issn.1672-7770.2012.03.002
经枕大池移植神经干细胞治疗重型颅脑损伤大鼠的实验研究
Therapeutic study of neural stem cells injected into cistern magna on the rat model of severe craniocerebral injury
刘建新 1王晓峰 1张荣军 1李拴德 1王小亚 1王瑞安2
作者信息
- 1. 721004 宝鸡,解放军第三医院神经外科研究所
- 2. 第四军医大学基础部
- 折叠
摘要
Abstract
Objective To observe the effect of fetal brain cortex derived neural stem cells transplantation on treating severe craniocerebral injury rats. Method 46 severe craniocerebral injury rats were generated by electronic craniocerebral injury instrument, and randomly divided into the neural stem cell therapy group ( n = 23 ) and the control group ( n = 23 ). After culturing for 3 days, the third generation passage cultured neural stem cells were labeled with BrdU, and injected into the occipital pool. The control group were injected with same volume of DMEM/F12. Two groups of rats were tested improved neurological deficit scores after injection on the 24 hours,3rd, 7th, 14th, 21" and 24'"' day. Four weeks after transplantation, five rats of the therapy group were sacrificed, and the BrdU-positive cells in brain were examined by immunohislochemical method. Result The results showed that after transplantation of neural stem cells, on the 7th, 14th , 21"1 and 24th day, the paralysis symptoms were greatly improved. The neurological deficit score of the therapy group were significantly different compared with the control group. A large number of BrdU-positive cells were observed around the foci of brain damage in the 5 sacrificed rats after four weeks transplantation. Conclusion Transplantation of cultured neural stem cells can remain alive and grow in the rat brain, and facilitate the functional recovery in craniocerebral injury rat.关键词
神经干细胞/颅脑损伤/移植Key words
neural stem cells/ craniocerebral injury/ transplantation分类
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刘建新,王晓峰,张荣军,李拴德,王小亚,王瑞安..经枕大池移植神经干细胞治疗重型颅脑损伤大鼠的实验研究[J].临床神经外科杂志,2012,9(3):133-135,3.
