临床神经外科杂志2012，Vol.9Issue(3)：136-138,3.DOI:10.3969/j.issn.1672-7770.2012.03.003
大鼠胎脑神经干细胞的培养分化及鉴定的实验研究
Experimental study of cultivation, differentiation and identification in fetal rats neural stem cells
刘建新 1王晓峰 1张荣军 1王瑞安 2李拴德 1王小亚1
作者信息
- 1. 721004 宝鸡,解放军第三医院神经外科研究所
- 2. 第四军医大学基础部
- 折叠
摘要
Abstract
Objective To establish methods of cultivation, amplification, differentiation and identification in fetal rats ' cortical neural stem cells ( NSC ). Method 14d fetal rats ' cerebral cortex was selected as source of cells. To establish the vitro system of embryo neural stem cells, the B27 , basic fibroblast growth factor and vascular endothelial growth factor were added in serum of free medium, 5% of the womb bovine serum was used for differentiation. Immune fluorescence staining tcchniques were used in identification of neural stem cells and differentiated cells. Results The isolated cultivation of neural stem cells had strong refraction, the culturcd cells began to form colonies from 2nd to 3rd which form large neurospheres. Cells of 3 to 5 passages grew stable. The neurospheres which inducted by fetal bovine serum,from 3rd,began to give prominence to the surrounding and suspension cells begin to grow adherent. The neurospheres of original and passage generation expressed Nestin positive, cells of differentiation expressed NSE, GFAP respectively and expressed GALC positive. Conclusion By using serum-free culture technology we can culture, amplify neural stem cells in vitro. 5% of the womb bovine serum can induce neural stem cells differentiate to neurons cells, astrocytes cells and oligodendrocytes cells and other mature cells.关键词
神经干细胞/体外培养/鉴定Key words
neural slem cells/cultivation in vitro/identification分类
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刘建新,王晓峰,张荣军,王瑞安,李拴德,王小亚..大鼠胎脑神经干细胞的培养分化及鉴定的实验研究[J].临床神经外科杂志,2012,9(3):136-138,3.
