环境与职业医学2012，Vol.29Issue(6)：343-346,4.
高效液相色谱-串联质谱法同时测定水产品中7种微囊藻毒素
Determination of 7 Microcystin Variants in Aquatic Products by High Performance Liquid ChromatographyTandem Triple Quadrupole Mass Spectrometry
摘要
Abstract
[ 0bjectiV8 ] To establish a high performance liquid chromatography-tandem triple quadrupole mass spectrometry method for simultaneous determination of 7 variants of microcystins in aquatic products. [ Methods J All samples were subject to extraction in Tris-HCl buffer (pH=7), clean-up with Oasis ⅡLB solid phase extraction, C18 separation, mass spectrometry detection and quantification with internal standards. [ Results ] After effective extraction and purification, 7 microcystins of LR, RR, YR, LA, LW, LY and LF were detected simultaneously. The mean recoveries spiked at levels of 10|ig/kg and 40μg/kg in four matrices were 51%-112% and 60%-117%, with relative standard deviation of 1.7%-12.7% and 0.9%-8.0% (n=6), respectively. The limits of detection were 0.20-0.40 μg/kg. [ Conclusion ] A rapid and practical method with high efficiency is proposed and provides technical basis for standard determination protocol of microcystins.关键词
微囊藻毒素/水产品/液相色谱-串联质谱/节球藻毒素Key words
microcystin/ aquatic products/ high performance liquid chromatography-tandem triple quadrupolemass spectrometry/ nodularin分类
生物科学引用本文复制引用
金玉娥,汪国权,马佳鸣,朱怡平,温忆敏,刘弘..高效液相色谱-串联质谱法同时测定水产品中7种微囊藻毒素[J].环境与职业医学,2012,29(6):343-346,4.基金项目
上海市科学技术委员会项目(编号:09411967200) （编号:09411967200）
