环境与职业医学2012，Vol.29Issue(6)：347-350,354,5.
中波紫外线致雄性小鼠生殖系统损伤
Ultraviolet-B Radiation Damage to Reproductive System in Mice
摘要
Abstract
[ Objective J To study possible damages of ultraviolet-B (UVB) to reproductive 9ystem of mice. [ Methods ] Thirty-six male mice were randomized into 3 groups: control group, 2h irradiated group and 3h irradiated group, each with 12 animals. The mice were sacrificed after 30 days of UVB irradiation (285 nm) and measured testicle to body weight ratio, sperm motility and malformation rates, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content, and recorded pathological changes. [ Results ] Compared with the control group, the body weights and the testicle to body weight ratios of the two irradiated groups were significantly declined (P<0.01); the sperm motility rates were significantly declined (P<0.05); the sperm malformation rates were increased but not significantly (P>0.05). The pathological changes in the testis samples showed: interstitial tissues swelled and were separated from convoluted tubules; spermatogenie epitheliums were loosely-arranged and fell into the tubules; tubules were narrowed; and the counts of sperm decreased. The damage in the 3 h irradiated group was more obvious. UVB radiation significantly and dose-dependently decreased SOD activity and raised MDA content in radiation groups as compared with the control group (P<0.01). [ Conclusion ] Radiation of UVB could decrease body weight and testicle to body weight ratio, cause pathological changes in the testis of mice, degrade SOD activity, raise MDA content, but couldn't affect sperm formation obviously. These injuries are possible results of reactive oxygen species (ROS) induced by UVB irradiation indirectly.关键词
中波紫外线/生殖系统/睾丸组织损伤/精子活率/畸形率/雄性小鼠Key words
ultraviolet-B/ reproductive system/ testicular tissue damage/ sperm motility rate/ malformation rate/male mice分类
医药卫生引用本文复制引用
杨明建,郑燕,申海玉,郭海燕,叶嘉,李艳杰..中波紫外线致雄性小鼠生殖系统损伤[J].环境与职业医学,2012,29(6):347-350,354,5.基金项目
邯郸市科学技术研究与发展计划项目(编号:0821107050-2) （编号:0821107050-2）
邯郸学院2007年度博士、硕士科研经费启动项目(编号:2007006) （编号:2007006）
