陈鹤峰 1李向阳 1缪应新 2甘洁民 2洪慰麟 1周瑾1
作者信息
- 1. 200040 上海市,复旦大学附属华东医院呼吸科
- 2. 200040 上海市,复旦大学附属华东医院中心实验室
- 折叠
摘要
Abstract
Objective To investigate the relationship between leptin receptor gene Gln223Arg polymorphism and malnutrition of patients with chronic obstructive pulmonary disease (COPD). Methods 158 elderly patients wilh COPD in stable stage and 10S normal controls were studied. Nutritional parameters, including body mass index (BMI), percentage of normal body mass (NM%), triceps skin-fold thickness (TSF), mid-upper arm circumference (MAC), serum album (ALB), total lymphocyte counts (LYM) in all cases, were evaluated, respectively. COPD patients were divided into malnutrition group (group 1) and non-malnutrition group (group 2) according to the nutrition parameter. Serum leptin levels were measured by ELISA. The frequencies of genotype and allele single nucleotide polymorphism of leptin receptor gene (Gln223Arg) were detected via with polymerase chain reaction-ligase detection reaction and compared. Results In patients of COPD with malnutrition, the frequencies of genotype GG, GA, and AA were 0.924, 0.061 and 0.015, respectively, while those in patients of COPD without malnutrition were 0.783, 0.206 and 0.011, respectively, whilethose in controls were 0.769, 0.222 and 0.009; The allele frequencies of G and A in patients of COPD with malnutrition were 0.955 and 0.045, while those in patients of COPD without malnutrition were 0.886 and 0.114, while those in controls were 0.88 and 0.12. Significant differences were detected in genotypes and allele frequencies of Gln223Arg between COPD 1 subjects, COPD 2 subjects and controls, but there was no obvious difference between COPD 2 subjects and controls. And there were no significant differences in leptin levels between GG, A/G and AA genotypes. Conclusion LEPR Gln223Arg polymorphism may associate with malnutrition of patients with COPD.关键词
慢性阻塞性肺疾病/营养不良/瘦素/瘦素受体/基因多态性Key words
Chronic obstructive pulmonary disease/ Malnutrition/ Leptin/ Leptin receptor/ Gene polymorphisms分类
医药卫生
